tlr9 antagonist odn 2088  (MedChemExpress)

Journal: Molecular and Cellular Biochemistry

Article Title: NETs accelerate aortic valve calcification by promoting M1 macrophage polarization through the TLR9 signaling pathway

doi: 10.1007/s11010-025-05375-z

Figure Lengend Snippet: The protein and mRNA expression of aortic valve tissue of mice in each group. TLR9: Toll-like receptor 9; RUNX2: Runt-related transcription factor 2; BMP2: Bone morphogenetic protein 2; TNF-α: Tumor necrosis factor-α; IL-10: Interleukin-10; A Aortic valve tissue protein immunoblotting ( n = 3 one-way analysis of variance); B TLR9 protein expression level in aortic valve tissue ( n = 3 one-way analysis of variance); C the expression level of RUNX2 protein in aortic valve tissue ( n = 3 one-way analysis of variance); D the expression level of BMP2 protein in aortic valve tissue ( n = 3 one-way analysis of variance); E the expression level of TNF-α protein in aortic valve tissue ( n = 3 one-way analysis of variance); F the expression level of IL-10 protein in aortic valve tissue ( n = 3 one-way analysis of variance); G RT-qPCR line chart of aortic valve tissue in each group ( n = 3 one-way analysis of variance); H BMP2 mRNA expression level in aortic valve tissue ( n = 3 one-way analysis of variance); I RUNX2 mRNA expression level in aortic valve tissue ( n = 3 one-way analysis of variance); J TLR9 mRNA expression level in aortic valve tissue ( n = 3 one-way analysis of variance); K the expression level of IL-1β mRNA in aortic valve tissue ( n = 3 one-way analysis of variance); L The expression level of IL-10 mRNA in aortic valve tissue ( n = 3 one-way analysis of variance); ns p > 0.05; * p < 0.05; ** p < 0.01; *** p < 0.001

Article Snippet: Mice peripheral neutrophils containing 10% vehicle group serum, containing 10% CAVD model group serum or treated with PMA, were incubated for 24 h. Their cell supernatants were treated with Raw264.7 cells for 24 h. The cells were incubated for 24 h with or without the TLR9 antagonist ODN2088 (1 μM, InvivoGen), and the TLR 9 agonist ODN1826 (1 μM, InvivoGen) was incubated for another 24 h, respectively.

Techniques: Expressing, Western Blot, Quantitative RT-PCR

Journal: Molecular and Cellular Biochemistry

Article Title: NETs accelerate aortic valve calcification by promoting M1 macrophage polarization through the TLR9 signaling pathway

doi: 10.1007/s11010-025-05375-z

Figure Lengend Snippet: Flow cytometry and RT-qPCR detection results and statistical figures. A CD86- and CD206-positive cells were detected by flow cytometry (3 biological replicates). B The proportion of CD86-positive cells (3 biological replicates). C The proportion of CD206-positive cells (3 biological replicates). D Raw264.7 cells RT-qPCR line chart (3 biological replicates). E The expression level of TNF-α mRNA in Raw264.7 cells (3 biological replicates). F The expression level of IL-1β mRNA in Raw264.7 cells (3 biological replicates). G The expression level of IL-6 mRNA in Raw264.7 cells (3 biological replicates). H The expression level of TLR9 mRNA in Raw264.7 cells (3 biological replicates). I The expression level of IL-10 mRNA in Raw264.7 cells (3 biological replicates); ns p > 0.05; * p < 0.05; ** p < 0.01; *** p < 0.001

Article Snippet: Mice peripheral neutrophils containing 10% vehicle group serum, containing 10% CAVD model group serum or treated with PMA, were incubated for 24 h. Their cell supernatants were treated with Raw264.7 cells for 24 h. The cells were incubated for 24 h with or without the TLR9 antagonist ODN2088 (1 μM, InvivoGen), and the TLR 9 agonist ODN1826 (1 μM, InvivoGen) was incubated for another 24 h, respectively.

Techniques: Flow Cytometry, Quantitative RT-PCR, Expressing